焦虑对心肌梗死大鼠血清炎症因子水平影响及双心方干预效应观察
Effect of anxiety on serum inflammatory factors after myocardial infarction in rats and observation on intervention effect of Shuangxin Prescription
- 北京中医药 2022年41卷第4期 页码：385-389
- 作者机构：
1.北京中医药大学第三附属医院心血管内科，北京 100029
2.北京中医药大学东方医院，北京 100078
3.北京中医药大学第三附属医院科技处，北京 100029
- 作者简介：
王帅，男，35岁，硕士，主治医师。研究方向：中医药防治心脑血管疾病。
肖若然，E-mail：xrr002007@163.com
- 基金信息：国家自然科学基金资助项目(81373590);北京中医药大学中青年教师项目(2018-JYBZZ-JS167)
DOI：10.16025/j.1674-1307.2022.04.009
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王帅，赵海滨，王威，等.焦虑对心肌梗死大鼠血清炎症因子水平影响及双心方干预效应观察［J］.北京中医药，2022，41（4）：385-389.
WANG Shuai,ZHAO Hai-bin,WANG Wei,et al.Effect of anxiety on serum inflammatory factors after myocardial infarction in rats and observation on intervention effect of Shuangxin Prescription[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(04):385-389.
王帅，赵海滨，王威，等.焦虑对心肌梗死大鼠血清炎症因子水平影响及双心方干预效应观察［J］.北京中医药，2022，41（4）：385-389. DOI： 10.16025/j.1674-1307.2022.04.009.
WANG Shuai,ZHAO Hai-bin,WANG Wei,et al.Effect of anxiety on serum inflammatory factors after myocardial infarction in rats and observation on intervention effect of Shuangxin Prescription[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(04):385-389. DOI： 10.16025/j.1674-1307.2022.04.009.
摘要
目的,2,探讨焦虑对心肌梗死大鼠炎症因子（NF-κB、TNF-α、IL-1β）的影响及双心方的干预效应。,方法,2,根据随机数字表法将SD大鼠分为假手术组、模型组、中药组、西药组，每组10只。除假手术组外，各组采用空瓶刺激法与前降支结扎手术建立焦虑合并心肌梗死复合模型，中药组予以双心方中药颗粒剂灌胃干预，西药组予氟哌噻吨美利曲辛片灌胃干预。超声心动图评价大鼠心功能，旷野试验评价大鼠活动能力，高架十字迷宫评价大鼠焦虑状态，外周血检测NF-κB、TNF-α、IL-1β水平。,结果,2,与假手术组相比，模型组、中药组、西药组左室射血分数均降低，中药组左室射血分数高于模型组、西药组，差异均有统计学意义（,P,<,0.05）。与假手术组相比，模型组、中药组、西药组血清NF-κB、TNF-α、IL-1β表达均增加，但中药组血清NF-κB、TNF-α、IL-1β表达低于模型组、西药组，差异均有统计学意义（,P,<,0.05）。与模型组相比，中药组、西药组均能改善大鼠焦虑行为。,结论,2,焦虑可影响心肌梗死大鼠炎症因子水平，而双心方干预可降低其血清TNF-α、IL-6、IL-1β表达，改善心功能，减轻焦虑行为。
Abstract
Objective,2,To investigate the effect of anxiety on inflammatory factors（NF-κB，TNF-，IL-1）after myocardial infarction in rats and the intervention effect of Shuangxin Prescription.,Methods,2,SD rats were divided into control group，model group，Chinese medicine group and western medicine group according to random number table，with 10 rats in each group. The composite model of anxiety combined with myocardial infarction was established by empty bottle stimulation and ligation of anterior descending branch，and intervention was given by intragastric administration of Shuangxin Prescription. Cardiac function was evaluated by echocardiography，activity ability by field test，anxiety state by elevated cross maze，and levels of NF-κB，TNF- and IL-1 in peripheral blood were detected.,Results,2,Compared with the control group，the left ventricular ejection fraction of the model group，the Chinese medicine group and the Western medicine group was lower than that of the myocardial infarction group，and the left ventricular ejection fraction of the Chinese medicine group was higher than that of the model group and the western medicine group，with statistical significance in difference（,P,<,0.05）. Compared with the control group，the expressions of NF-κB，TNF- and IL-1 in serum of model group，Chinese medicine group and western medicine group were increased，but the expressions of NF-κB，TNF- and IL-1 in serum of Chinese medicine group were lower than those in model group and western medicine group，the differences were statistically significant （,P,<,0.05）. Compared with the model group，the anxiety behavior was improved in both the Chinese medicine group and the western medicine group. C,onclusion ,Anxiety can affect the level of inflammatory factors of rats with myocardial infarction and Shuangxin Prescription can reduce the expression of TNF-，IL-6 and IL-1 in serum and promote the cardiac function and relieve anxiety behavior.
关键词
双心方心肌梗死炎症因子焦虑大鼠
Keywords
Shuangxin Prescriptionmyocardial infarctioninflammatory factoranxietyrat
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