Objective,2,To study the effects of Tangluoning on IRE1α-CHOP signaling pathway in rat dorsal root neurons（DRGn）under high glucose environment.,Methods,2,Sixty clean grade male SD rats were randomly divided into Tangluoning group（25 rats）and normal group（35 rats），and the crude drug of Tangluoning was given in the Tangluoning group with 2.5 g/（kg•d）and the same amount of distilled water was administered by gavage for the preparation of comparative serum.The dorsal root ganglion（DRG）used to prepare cell suspension was obtained from SD fetal rats.DRGn cells were inoculated into 6-well plate and randomly divided into 7 groups：normal group（group A cultured with blank serum），model group（group B cultured with 75 mmol/L glucose and blank serum），Tangluoning group（group C cultured with 75 mmol/L glucose+Tangluoning medicated serum），miR⁃211 inhibitor group（group D cultured 75 mmol/L glucose+blank serum culture+miR⁃211 inhibitor negative），miR⁃211 inhibitor control group（group d cultured with 75 mmol/L glucose+Tangluoning medicated serum culture+miR⁃211 inhibitor negative control），agonist traditional Chinese medicine group（group E cultured with 75 mmol/L glucose+Tangluoning medicated serum culture+miR⁃211 agonist）and agonist traditional Chinese medicine control group（group e cultured with 75 mmol/L glucose+Tangluoning medicated serum culture+miR⁃211 agonist negative control）.After 24 hours of culture，q⁃PCR was used to detect the gene expression levels of miR⁃211，IRE1α，p⁃IRE1α，XBP1 and CHOP in DRGn cells；Fluorescence probe method was used to detect ROS expression in DRGn cells，xanthine oxidase technique was used to detect SOD activity in DRGn cells，and thiobarbital method was used to detect MDA level.,Results,2,Compared the levels of ROS，SOD，MDA，miR⁃211 mrna，IRE1α，XBP1 and CHOP in each group，the differences were statistically significant（,P,<,0.01）.Compared with group A，the levels of ROS，MDA as well as miR⁃211，IRE1α，XBP1，CHOP mRNA in group B were obviously heightened and SOD reduced obviously（,P,<,0.01）.Compared with group B，the levels of ROS，MDA as well as miR⁃211，IRE1α，XBP1，CHOP mRNA in group C and D were obviously reduced and SOD level of group C was higher than that of group B.Compared the levels of ROS，MDA as well as miR⁃211，IRE1α，XBP1，CHOP mRNA in group d with B，the differences were not statistically significant（,P,>,0.05）；compared with group B，the levels of ROS，SOD，MDA，miR⁃211，IRE1α，XBP1，CHOP mRNA in group E were obviously heightened，SOD reduced（,P,<,0.01）；compared the levels of ROS，SOD，MDA，miR⁃211，IRE1α，XBP1，CHOP mRNA in group e with group C，the differences were not statistically significant（,P,>,0.05）.,Conclusion,2,Tangluoning could reduce the activity of IRE1α-CHOP pathway by down-regulating the expression of miR⁃211.