大黄素对多囊卵巢综合征大鼠卵巢功能及性激素水平的影响
Effect of emodin on ovarian function and sex hormone level regulation in rats with polycystic ovary syndrome
- 北京中医药 2022年41卷第7期 页码：738-742
- 作者机构：
1.河南省生殖妇产医院检验科，郑州 450000
- 作者简介：
杨永俊，男，50岁，大学本科，副主任医师。研究方向：中西医结合计划生育医学。
- 基金信息：河南省人口和计划生育科学技术研究院基本科研业务项目(JBKY2017004)
DOI：10.16025/j.1674-1307.2022.07.008
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杨永俊，李铭，曹丽幸，等.大黄素对多囊卵巢综合征大鼠卵巢功能及性激素水平的影响［J］.北京中医药，2022，41（7）：738-742.
YANG Yong-jun,LI Ming,CAO Li-xing,et al.Effect of emodin on ovarian function and sex hormone level regulation in rats with polycystic ovary syndrome[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(07):738-742.
杨永俊，李铭，曹丽幸，等.大黄素对多囊卵巢综合征大鼠卵巢功能及性激素水平的影响［J］.北京中医药，2022，41（7）：738-742. DOI： 10.16025/j.1674-1307.2022.07.008.
YANG Yong-jun,LI Ming,CAO Li-xing,et al.Effect of emodin on ovarian function and sex hormone level regulation in rats with polycystic ovary syndrome[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(07):738-742. DOI： 10.16025/j.1674-1307.2022.07.008.
摘要
目的,2,探讨大黄素对多囊卵巢综合征（PCOS）大鼠的卵巢功能及性激素水平的影响。,方法,2,选择性周期规律的未孕雌性SD大鼠28只，随机分为空白对照组、对照+大黄素组、PCOS模型组、模型+大黄素组，各7只。PCOS模型组、模型+大黄素组于大鼠背部肌内注射4 mg戊酸雌二醇制备PCOS模型；诱导后，对照+大黄素组、模型+大黄素组大鼠腹腔内注射大黄素25 mg/kg；空白对照组及PCOS模型组大鼠注射等量生理盐水。均1次/d，连续14 d。每周测量各组大鼠体质量。干预14 d后，经大鼠尾静脉采集外周血，分离获得血清后进行相关检测。脱颈法处死大鼠后收集子宫和卵巢组织样本，分别测量质量。测定大鼠性周期，计算卵巢质量/体质量、子宫质量/体质量，采用HE染色法观察卵巢和子宫组织学变化，采用ELISA法检测血清睾酮及抗缪勒氏管激素（AMH）。,结果,2,与空白对照组比较，对照+大黄素组发情间期无明显改变（,P,>,0.05），PCOS模型组发情间期比例高（,P,<,0.05）；与PCOS模型组比较，模型+大黄素组发情间期比例低（,P,<,0.05）。与空白对照组比较，对照+大黄素组大鼠卵巢质量/体质量无明显改变（,P,>,0.05），PCOS模型组卵巢质量/体质量高（,P,<,0.05）；与PCOS模型组比较，模型+大黄素组卵巢质量/体质量低（,P,<,0.05）。与空白对照组比较，对照+大黄素组大鼠子宫质量/体质量无明显改变（,P,>,0.05），PCOS模型组子宫质量/体质量低（,P,<,0.05）；与PCOS模型组比较，模型+大黄素组子宫质量/体质量高（,P,<,0.05）。PCOS组出现1个大的充液性囊性卵泡，其颗粒层凋亡脱落，间膜层形成上皮样细胞增生内壁，囊液中含有巨噬细胞。模型+大黄素组却未观察到该现象，而且未观察到卵泡增大的趋势。与空白对照组比较，PCOS模型组血清雄激素水平高（,P,<,0.05）；与PCOS模型组比较，模型+大黄素组大鼠血清雄激素水平低（,P,<,0.05）。与空白对照组比较，PCOS模型组血清AMH水平高（,P,<,0.05）；与PCOS模型组比较，模型+大黄素组AMH水平低（,P,<,0.05）。,结论,2,大黄素可改善PCOS大鼠的卵巢功能，使各性激素水平保持平衡。
Abstract
Objective,2,To evaluate the effect of emodin on ovarian function and sex hormone level in rats with polycystic ovary syndrome.,Methods,2,Twenty-eight non-pregnant female Sprague Dawley rats with periodic regularity were randomly divided into four groups（7 rats/group），including control group，control+emodin group，polycystic ovary syndrome（PCOS）model group and model+emodin treatment group.PCOS model group and model+emodin group were injected with 4 mg estradiol valerate into the back muscle of rats to prepare PCOS model.After induction，rats in control+emodin group and model+emodin group were injected with emodin 25 mg/kg intraperitoneally.Rats in control group and PCOS model group were injected with the same amount of normal saline.All these groups were treated once a day for 14 days.The body weight of rats in each group was measured every week.After 14 days of treatment，peripheral blood was collected from tail vein of rats in each group，and serum was obtained by centrifugal separation for the related tests.After the rats in each group were killed by decapitation，samples of uterus and ovary tissues were collected，and the weights of ovaries and uterus were measured respectively.The sexual cycle of rats was measured，the ovarian mass/body mass and uterine mass/body mass were calculated，the histological changes of ovaries and uterus were observed by HE staining，and the levels of testosterone and anti Mullerian hormone（AMH）in plasma were measured.,Results,2,Compared with the blank control group，the estrus interval of the control+emodin group had no significant change（,P,>,0.05）.Compared with the blank control group，the proportion of estrus interval in PCOS model group was higher（,P,<,0.05）.Compared with PCOS model group，the proportion of estrus interval in model+emodin group was lower（,P,<,0.05）.Compared with the blank control group， the ovarian mass/body mass of rats in the control+emodin group had no significant change（,P,>,0.05）.Compared with the blank control group，the ovarian mass/body mass of PCOS model group was higher（,P,<,0.05）.Compared with PCOS model group，the ovarian mass/body mass of model+emodin group was lower（,P,<,0.05）.Compared with the blank control group，the uterine mass/body mass of rats in the control+emodin group had no significant change（,P,>,0.05）.Compared with the blank control group，the uterine mass/body mass of PCOS model group was lower（,P,<,0.05）.Compared with PCOS model group，the uterine mass/body mass of model+emodin group was higher（,P,<,0.05）.In PCOS group，a large fluid-filled cystic follicle appeared，its granular layer died off，and its membranous layer formed the inner wall of epithelial-like cell proliferation，and the cystic fluid contained macrophages.This phenomenon was not observed in the model+emodin group，and the tendency of follicle enlargement was not observed.Compared with the blank control group，the serum androgen level in PCOS model group was higher（,P,<,0.05）.Compared with PCOS model group，serum androgen level in model+emodin group was lower（,P,<,0.05）.Compared with the blank control group，the serum AMH level in PCOS model group was higher（,P,<,0.05）.Compared with PCOS model group，AMH level in model+emodin group was lower（,P,<,0.05）.,Conclusion,2,Emodin can improve the ovarian function of PCOS rats and keep the balance of the levels of various sex hormones.
关键词
大黄素多囊卵巢综合征卵巢功能性激素大鼠
Keywords
Emodinpolycystic ovary syndromeovarysex hormonesrats
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