清热化湿祛瘀方对轻中度溃疡性结肠炎患者肠道菌群的影响
Effect of Qingre Huashi Quyu Recipe on intestinal flora in patients with mild to moderate ulcerative colitis
- 北京中医药 2022年41卷第9期 页码：963-967
- 作者机构：
1.首都医科大学附属北京中医医院消化中心，100010
2.北京中医药大学，100029
- 作者简介：
邓鑫，女，28岁，博士研究生。研究方向：中医药治疗脾胃病。
赵鲁卿，E-mail：zhaoluqing@bjzhongyi.com
- 基金信息：北京市科技新星计划项目(Z201100006820119)
DOI：10.16025/j.1674-1307.2022.09.005
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邓鑫，王澍青，窦丹，等.清热化湿祛瘀方对轻中度溃疡性结肠炎患者肠道菌群的影响［J］.北京中医药，2022，41（9）：963-967.
DENG Xin,WANG Shu-qing,KOU Dan,et al.Effect of Qingre Huashi Quyu Recipe on intestinal flora in patients with mild to moderate ulcerative colitis[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(09):963-967.
邓鑫，王澍青，窦丹，等.清热化湿祛瘀方对轻中度溃疡性结肠炎患者肠道菌群的影响［J］.北京中医药，2022，41（9）：963-967. DOI： 10.16025/j.1674-1307.2022.09.005.
DENG Xin,WANG Shu-qing,KOU Dan,et al.Effect of Qingre Huashi Quyu Recipe on intestinal flora in patients with mild to moderate ulcerative colitis[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(09):963-967. DOI： 10.16025/j.1674-1307.2022.09.005.
摘要
目的,2,观察清热化湿祛瘀方对轻中度溃疡性结肠炎（UC）患者肠道菌群的影响。,方法,2,选取2018年6月―2021年8月首都医科大学附属北京中医医院收治的13例UC活动期患者为研究对象，患者在长期美沙拉嗪基础治疗上给予清热化湿祛瘀方，连续治疗12周，通过16S rRNA测序技术检测治疗前、后肠道菌群的分布，观察清热化湿祛瘀方对肠道菌群的调控作用。,结果,2,清热化湿祛瘀方治疗后菌群多样性及丰富度均较前升高；厚壁菌门（Firmicutes）、普拉梭菌属（Faecalibacterium）丰度均有所升高，且拟杆菌门（Bacteriodetes）、大肠杆菌属（Escherichia）丰度有所下降。,结论,2,清热化湿祛瘀方治疗有助于增加肠道菌群的多样性和物种丰富度，同时可辅助有益菌的增长，并抑制致病菌的增殖，从而更好地维持肠道微环境。
Abstract
Objective,2,To observe the effect of Qingre Huashi Quyu Recipe on intestinal flora in patients with mild to moderate ulcerative colitis（UC）.,Methods,2,13 patients with UC in the active stage who were admitted to the Beijing Traditional Chinese Medicine Hospital Affiliated to the Capital Medical University from June 2018 to August 2021 were selected as the research objects. The patients were treated with Qingre Huashi Quyu Recipe for 12 weeks on the basis of long-term mesalazine treatment. The distribution of intestinal flora before and after treatment was detected by 16s rRNA sequencing technology， and the regulatory action of Qingre Huashi Quyu Recipe on intestinal flora was observed.,Results,2,The diversity and abundance of flora after the treatment were higher than before；the abundance of Firmicutes and Faecalibacterium was increased， while the abundance of Bacteroides and Escherichia coli decreased.,Conclusion,2,Qingre Huashi Quyu Recipe can help to increase the diversity and species richness of intestinal flora，assist the growth of beneficial bacteria，and inhibit the proliferation of pathogenic bacteria，so as to make a better maintainence of the intestinal microenvironment.
关键词
溃疡性结肠炎清热化湿祛瘀方肠道菌群16S rRNA
Keywords
Ulcerative colitisQingre Huashi Quyu Recipeintestinal flora16S rRNA
references
LEE M,CHANG EB.Inflammatory bowel diseases(IBD)and the microbiome-searching the crime scene for clues[J].Gastroenterology,2021,160(2):524-537.
周正华,冀建斌.中医药整体与局部相结合辨治溃疡性结肠炎[J].北京中医药,2020,39(3):196-199.
沈洪,邢敬,朱磊,等.炎症性肠病中西医结合治疗策略的探索与实践[J].北京中医药,2020,39(3):191-195.
苏晓兰,国嵩,张涛,等.炎症性肠病诊治现状及中医药治疗特色与优势[J]. 北京中医药,2020,39(3):211-215.
俞媛,李晨.芍黄安肠汤对溃疡性结肠炎患者肠道菌群及氧化应激的影响[J].北京中医药,2019,38(9):927-930.
TRUELOVE SC,WITTS LJ.Cortisone in ulcerative colitis:final report on a therapeutic trial[J].Br Med J,1955,2:1041-1048.
张声生，沈洪，郑凯，等.溃疡性结肠炎中医诊疗专家共识意见(2017)[J].中华中医药杂志,2017,32(8):3585-3589.
GUO XY,LIU XJ,HAO JY.Gut microbiota in ulcerative colitis:insights on pathogenesis and treatment[J].J Dig Dis,2020,21(3):147-159.
丁庞华,李军祥,郭一,等.基于高通量测序技术的溃疡性结肠炎大肠湿热证患者肠道菌群多样性的研究[J].世界科学技术-中医药现代化,2018,20(6):967-973.
赵鲁卿,吴茜,张声生.张声生教授以“寒热”为纲辨治溃疡性结肠炎的思路和治疗策略[J].中国中西医结合消化杂志,2021,29(2):141-143.
靳瑾,周知然.黄连解毒汤对溃疡性结肠炎患者肠道菌群的影响[J].中医学报,2020,35(7):1520-1523，1559.
段学清,陈瑞,朱晨,等.黄连对大鼠肠道菌群的影响[J].时珍国医国药,2021,32(5):1065-1070.
刘世锋,罗玉霜,刘佩琳,等.黄芩提取物对人体肠道菌群及短链脂肪酸的影响[J].中国食物与营养,2021,27(1):49-53.
高珊,柳清.氧化苦参碱通过调节肠道菌群抑制小鼠结直肠癌的相关研究[J].肿瘤研究与临床,2021,33(9):645-650.
MACHIELS K,JOOSSENS M,SABINO J,et al.A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis[J].Gut,2014,63(8):1275-1283.
PARADA VENEGAS D,DE LA FUENTE MK,LANDSKRON G,et al.Short chain fatty acids(SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases[J].Front Immunol,2019,10:277.
RATH HC,HERFARTH HH,IKEDA JS,et al.Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats[J].J Clin Invest,1996,98(4):945-953.
OHKUSA T,YOSHIDA T,SATO N,et al.Commensal bacteria can enter colonic epithelial cells and induce proinflammatory cytokine secretion:a possible pathogenic mechanism of ulcerative colitis[J].J Med Microbiol,2009,58(Pt5):535-545.
ARUMUGAM M,RAES J,PELLETIER E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473(7346):174-180.
CARLSSON AH,YAKYMENKO O,OLIVIER I,et al.Faecalibacterium prausnitzii supernatant improves intestinal barrier function in mice DSS colitis[J].Scand J Gastroenterol,2013,48(10):1136-1144.
LOUIS P,FLINT HJ.Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine[J].FEMS Microbiol Lett,2009,294(1):1-8.
BURKE DA,AXON AT.Adhesive Escherichia coli in inflammatory bowel disease and infective diarrhoea[J].BMJ,1988,297(6641):102-104.
BURKE DA,AXON AT.Ulcerative colitis and Escherichia coli with adhesive properties[J].J Clin Pathol,1987,40(7):782-786.
MIRSEPASI-LAURIDSEN HC,DU Z,STRUVE C,et al. Secretion of Alpha-Hemolysin by Escherichia coli disrupts tight junctions in ulcerative colitis patients[J].Clin Transl Gastroenterol,2016,7(3):e149.
JENSEN SR,MIRSEPASI-LAURIDSEN HC,THYSEN AH,et al.Distinct inflammatory and cytopathic characteristics of Escherichia coli isolates from inflammatory bowel disease patients[J].Int J Med Microbiol,2015,305(8):925-936.
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