基于PGC-1α/NRF-1/Tfam探讨芪珀生脉颗粒对HL-1心房肌细胞线粒体生物合成的改善作用

石树青; 殷仕洁; 张雪松; 杜宜航; 胡元会

doi:10.16025/j.1674-1307.2022.10.003

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基于PGC-1α/NRF-1/Tfam探讨芪珀生脉颗粒对HL-1心房肌细胞线粒体生物合成的改善作用

专题 | 更新时间：2023-06-16

- 基于PGC-1α/NRF-1/Tfam探讨芪珀生脉颗粒对HL-1心房肌细胞线粒体生物合成的改善作用
- Improvement effect of Qipo Shengmai Granules on mitochondrial biosynthesis in HL-1 atrial myocytes based on PGC-1α/NRF-1/Tfam
- 北京中医药 2022年41卷第10期页码：1084-1090
- 作者机构：
  
  1.中国中医科学院，北京 100007
  2.中国医学科学院阜外医院中医科，北京 100037
  3.中国中医科学院广安门医院心血管科，北京 100053
  4.中国中医科学院广安门医院，北京 100053
- 作者简介：
  
  石树青，女，32岁，博士，主治医师。研究方向：中医药防治心血管疾病。
  胡元会，E-mail：huiyuhui55@sohu.com
- 基金信息：
  
  中央级公益性科研院所基本科研业务费专项(ZZ15-XY-LCQ-02);首都卫生发展科研专项(首发2022-1-4153);中国中医科学院科技创新工程项目(CI2021A00918)
- DOI：10.16025/j.1674-1307.2022.10.003
  中图分类号：
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石树青，殷仕洁，张雪松，等.基于PGC-1α/NRF-1/Tfam探讨芪珀生脉颗粒对HL-1心房肌细胞线粒体生物合成的改善作用［J］.北京中医药，2022，41（10）：1084-1090.

SHI Shu-qing,YIN Shi-jie,ZHANG Xue-song,et al.Improvement effect of Qipo Shengmai Granules on mitochondrial biosynthesis in HL-1 atrial myocytes based on PGC-1α/NRF-1/Tfam[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1084-1090.
石树青，殷仕洁，张雪松，等.基于PGC-1α/NRF-1/Tfam探讨芪珀生脉颗粒对HL-1心房肌细胞线粒体生物合成的改善作用［J］.北京中医药，2022，41（10）：1084-1090. DOI： 10.16025/j.1674-1307.2022.10.003.

SHI Shu-qing,YIN Shi-jie,ZHANG Xue-song,et al.Improvement effect of Qipo Shengmai Granules on mitochondrial biosynthesis in HL-1 atrial myocytes based on PGC-1α/NRF-1/Tfam[J]. Beijing Journal of Traditional Chinese Medicine,2022,41(10):1084-1090. DOI： 10.16025/j.1674-1307.2022.10.003.

摘要

目的,2,研究芪珀生脉颗粒含药血清改善快速起搏诱导的HL-1心肌细胞线粒体功能障碍的机制。,方法,2,运用电刺激HL-1心房肌细胞，在体外建立快速起搏的心房颤动细胞模型。随后采用转染小干扰RNA（siRNA）沉默PGC-1α基因表达，并给予芪珀生脉颗粒含药血清或空白血清干预，通过检测线粒体数量、ATP合成能力及线粒体生物合成相关蛋白的表达，观察在PGC-1α基因沉默时，芪珀生脉颗粒是否能发挥对心肌细胞线粒体的保护及促进合成作用。,结果,2,芪珀生脉颗粒含药血清可以提高快速起搏的HL-1心肌细胞线粒体数量、ATP含量，上调线粒体生物合成相关的蛋白，转染PGC-1α siRNA后，芪珀生脉颗粒的线粒体保护作用被阻断。,结论,2,芪珀生脉颗粒通过PGC-1α/NRF-1/Tfam介导的线粒体生物合成改善快速起搏HL-1心肌细胞线粒体功能障碍。

Abstract

Objective,2,To examine the improvement mechanism of mitochondrial biosynthesis in HL-1 cardiomyocytes mediated by fast pacing with Qipo Shengmai Granules （QPSM） contained serum.,Methods,2,An atrial fibrillation cell model with fast pacing was established in vitro by electrical stimulation of HL-1 atrial myocytes. Subsequently， the expression of PGC-1α gene was silenced by transfection of small interfering RNA （siRNA）， and were treated with QPSM-contained serum or blank serum. By detecting the number of mitochondria， ATP synthesis ability and the expression of mitochondrial biosynthesis-related proteins， it was observed that when PGC-1α gene was silenced， whether QPSM could still play a role in protecting and promoting the synthesis of cardiomyocyte mitochondria.,Results,2,The QPSM-contained serum could increase the number of mitochondria and ATP content， and up-regulate the proteins related to mitochondrial biosynthesis in rapidly pacing HL-1 cardiomyocytes. After transfection with PGC-1α siRNA， the mitochondrial protective effect of QPSM was blocked.,Conclusion,2,It is suggeted that QPSM may improve mitochondrial malfunction of fast paced HL-1 cardiomyocytes through PGC-1/NRF-1/Tfam-mediated mitochondrial biosynthesis.

关键词

芪珀生脉颗粒线粒体生物合成HL-1心肌细胞PGC-1α

Keywords

Qipo Shengmai Granulesmitochondrial biosynthesisHL-1 myocardial cellPGC-1α

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