Objective,2,To observe the effect of Qingre Jianpi Tiaochang Recipe in mice with DSS-induced colitis and the expression of spleen T lymphocytes， and to explore the mechanism of the recipe in treating DSS-induced colitis.,Methods,2,36 SPF C57BL/6 mice were selected and divided into normal group， model group， control group， high， medium， and low doses of Chinese medicine treatment group with 6 in each group. Except the normal group， the other groups of mice were given 2.5% DSS solution to induce ulcerative colitis model. After the molding is finished， the three Chinese medicine treatment groups were intragastrically administered with high， medium， and low doses of Qingre Jianpi Tiaochang Recipe， the control group was intragastrically administered with Salazine sustained-release granule suspension 0.52 g/（kg·d）， the normal and model groups were intragastrically administered with an equal volume of physiological saline. All groups got intervention for 7 days. Food intake， body weight， DAI， colon length and histopathology of mice in each group were observed. Serum levels of cytokines TNF-α， IL-10 and C-reactive protein （CRP） were determined by Elisa.The expression of Treg， Th17， CD4,+, and CD8,+, cells in mouse spleen was detected by flow cytometry.,Results,2,Food intake and body weight were increased， colonic morphology restored， the pathological injury improved and DAI reduced， the proportion of Treg and CD4,+, cells in spleen of mice increased， while the proportion of Th17 and CD8,+, cells decreased in the medium and high doses of Chinese medicine groups. Compared with model group， the difference was not statistically significant （,P,>,0.05）； compared with the model group， the serum TNF-α and CRP contents were lower （,P,<,0.05） and the IL-10 content was higher （,P,<,0.05） in the medium and high dose groups of Chinese medicine.,Conclusion,2,Qingre Jianpi Tiaochang Recipe can restore the body weight， increase food intake， decrease DAI score and reduce intestinal mucosal injury in UC mice.The mechanism may be mediated by regulating the expression and balance of spleen T lymphocytes and related cytokines in UC mice， so as to play an immunomodulatory role.