Objective

2

To investigate the effects of Bushen Huoxue Recipe on intestinal function and intestinal barrier on Parkinson's disease model mice.

Methods

2

72 C57 mice were randomly divided into normal group， model group， Madopar group， Bushen Huoxu Recipe low-dose group （BSHX-L group）， Bushen Huoxu Recipe medium-dose group （BSHX-M group） and Bushen Huoxu Recipe high-dose group （BSHX-H group）， with 12 mice in each group. Apart from the normal group， mice in other groups were intraperitoneally injected MPTP to induce PD model， BSHX-Lgroup， BSHX-M group and BSHX-H group were given grude herbs with 0.15， 0.3， 0.6 g/d， for two weeks consecutively； Madopar group was given Madopar 112.5mg/（kg·d） intraperitoneally， once a day for two weeks consecutively； the normal group and model group were given distilled water by intragastric administration， once a day for 14 consecutive days. The general condition and behavioral test （total open-pit movement distance， average open-pit movement speed and open-pit static time）， intestinal function test （fecal water content， small intestine charcoal powder propulsion rate， and 24 h defecation granule number）， the pathological morphology of colon and serum DAO and D-LA levels were detected and compared among the groups.

Results

2

Compared with the blank group， the mice in the model group showed listless spirit， dry and hard stool grains and decreased defecation volume， and the total active distance of open field was significantly reduced， the average active speed of open field was significantly slowed down， and the rest time of open field was significantly prolonged. The fecal water content and small intestine charcoal thrust rate in the model group were significantly decreased. The number of grains in 24 hours of defecation decreased （

P

<

0.05）； Compared with the model group， the Metoba group showed abnormal agility and increased fighting times after treatment， while the above conditions were improved after treatment in all Bushen Huoxue Ricipe groups. The total active distance of open field increased significantly， the avera

ge active speed of open field increased， and the rest time of open field decreased in Metoba group and Bushen Huoxue Recipe groups. The rate of small intestine carbon dust advancing in Metoba group increased significantly . Fecal water content and 24-hour defecation granules were increased in Bushen Huoxue Recipe high-dose group （

P

<

0.05）， inflammatory cell infiltration was improved in Metoba group and BSHX-L group， but intestinal crypt structure was still damaged. In BSHX-M group and BSHX-H group， the surface of intestinal crypts tended to be flat， the structure of glands and intestinal crypts recovered， and the degree of inflammatory cell infiltration decreased significantly. DAO and D-LA levels in Metoba group and Bushen Huoxue Recipe all dose groups were significantly decreased （all

P

<

0.05）.

Conclusion

2

Bushen Huoxue Recipe （tonifying kidney and promoting blood circulation） can significantly improve exercise state and intestinal function of PD model mice， and reduce the damage of colonic tissue structure and intestinal mucosal barrier.