芪蛭通窍胶囊对D-半乳糖诱导的大鼠阴茎海绵体平滑肌细胞衰老模型收缩功能的干预作用

颜俊锋; 姜卓呈; 叶妙勇; 郁建迪; 马利民; 张春和; 赵凡

doi:10.16025/j.1674-1307.2025.01.003

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芪蛭通窍胶囊对D-半乳糖诱导的大鼠阴茎海绵体平滑肌细胞衰老模型收缩功能的干预作用

专题——中医药治疗勃起功能障碍 | 更新时间：2025-01-24

- 芪蛭通窍胶囊对D-半乳糖诱导的大鼠阴茎海绵体平滑肌细胞衰老模型收缩功能的干预作用
- Intervention effect of Qizhi Tongqiao Capsules on the contractile function of the corpus cavernosum smooth muscle cells aging model in rats induced by D-galactose
- 北京中医药 2025年44卷第1期页码：12-18
- 作者机构：
  
  1.浙江大学医学院附属浙江医院泌尿外科，杭州 310030
  2.南通大学附属医院泌尿外科，南通 226001
  3.温州医科大学附属温岭医院泌尿外科，温岭 317500
  4.云南省中医医院泌尿男科，昆明 650021
- 作者简介：
  
  颜俊锋，男，33岁，硕士，主治医师。研究方向：勃起功能障碍的临床和基础研究。
  赵凡，E-mail：zhaofan@ntu.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(82104855;82274533;82004360);国家中西医协同“旗舰”科室（泌尿外科）建设项目(国中医药综结合函〔2024〕221号);中华中医药学会青年人才托举工程项目(CACM-2023-QNRC2-B21);江苏省研究型医院资助基金(YJXYY202204-YSA05;YJXYY202204-XKB08);南通市卫生健康委员会科研课题计划项目(MS2023020)
- DOI：10.16025/j.1674-1307.2025.01.003
  中图分类号：
- 纸质出版日期：2025-01-25，
  
  收稿日期：2024-09-28，
- 稿件说明：
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颜俊锋, 姜卓呈, 叶妙勇, 等. 芪蛭通窍胶囊对D-半乳糖诱导的大鼠阴茎海绵体平滑肌细胞衰老模型收缩功能的干预作用[J]. 北京中医药, 2025,44(1):12-18.

YAN JUNFENG, JIANG ZHUOCHENG, YE MIAOYONG, et al. Intervention effect of Qizhi Tongqiao Capsules on the contractile function of the corpus cavernosum smooth muscle cells aging model in rats induced by D-galactose. [J]. Beijing journal of traditional chinese medicine, 2025, 44(1): 12-18.
颜俊锋, 姜卓呈, 叶妙勇, 等. 芪蛭通窍胶囊对D-半乳糖诱导的大鼠阴茎海绵体平滑肌细胞衰老模型收缩功能的干预作用[J]. 北京中医药, 2025,44(1):12-18. DOI： 10.16025/j.1674-1307.2025.01.003.

YAN JUNFENG, JIANG ZHUOCHENG, YE MIAOYONG, et al. Intervention effect of Qizhi Tongqiao Capsules on the contractile function of the corpus cavernosum smooth muscle cells aging model in rats induced by D-galactose. [J]. Beijing journal of traditional chinese medicine, 2025, 44(1): 12-18. DOI： 10.16025/j.1674-1307.2025.01.003.

摘要

目的

探讨芪蛭通窍胶囊（QTC）对D-半乳糖诱导的原代大鼠阴茎海绵体平滑肌细胞（CCSMC）衰老模型收缩功能的影响及作用机制。

方法

体外分离、培养SD大鼠CCSMC，制备QTC含药血清。将CCSMC随机分为正常组、模型组、干预组。正常组常规培养，模型组、干预组在此基础上加入灭菌后的30 mg/mL的D-半乳糖溶液干预48 h制备衰老模型。正常组、模型组培养基中加入20% FBS，干预组培养基中加入20%的QTC含药血清，继续培养48 h。β-半乳糖苷酶染色实验观察CCSMC衰老状态，胶原凝胶收缩实验检测CCSMC收缩率，Western blotting法检测CCSMC表型相关蛋白［α-平滑肌激动蛋白（α-SMA）、结蛋白（Desmin）、I型胶原蛋白（Collagen I）、波形蛋白（Vimentin）］和Janus激酶2（JAK2）、信号转导和转录激活因子3（STAT3）蛋白表达。

结果

与正常组比较，模型组衰老阳性细胞数量多（

0.01）。与模型组比较，干预组阳性细胞数量少（

0.01）。与正常组比较，模型组CCSMC收缩率低（

0.01）。与模型组比较，干预组CCSMC收缩率高（

0.01）。与正常组比较，模型组CCMSC中α-SMA、Desmin、Collagen I、Vimentin、磷酸化JAK2（p-JAK2）/JAK2、磷酸化STAT3（p-STAT3）/STAT3表达高（

0.01）。与模型组比较，干预组CCMSC中α-SMA、Desmin、Collagen I、Vimentin、p-JAK2/JAK2、p-STAT3/STAT3表达低（

0.01）。

结论

QTC可促进衰老CCSMC表型变化，提高其收缩功能，其机制可能与JAK2/STAT3信号通路有关。

Abstract

Objective

To investigate the effect of Qizhi Tongqiao Capsules （QTC） on the contractile function of D-galactose-induced primary corpus cavernosum smooth muscle cells （CCSMC） aging model in rats and to explore the mechanism of action.

Methods

CCSMCs were isolated and cultured from SD rats in vitro， and QTC-containing serum was prepared. CCSMCs were randomly divided into the normal group， model group， and intervention group. The normal group was cultured conventionally， while the model and intervention groups were treated with sterilized 30 mg/mL D-galactose solution for 48 hours to induce aging. The normal and model groups were cultured with 20% FBS， while the intervention group was cultured with 20% QTC-containing serum for another 48 hours. The aging state of CCSMCs was observed by β-galactosidase staining， and the contractile rate was measured by a collagen gel contraction assay. The expression of phenotypic proteins ［α-smooth muscle actin （α-SMA）， desmin， collagen I， vimentin］， Janus kinase 2 （JAK2）， and signal transducer and activator of transcription protein 3 （STAT3） was detected by Western blot.

Results

Compared with the normal group， the model group exhibited a significantly higher number of positive cells （

0.01）. Compared with the model group， the intervention group showed a significantly lower number of positive cells （

0.01）. The contractile rate of CCSMCs in the model group was significantly lower than that of the normal group （

0.01）. Compared with the model group， the intervention group exhibited a significantly higher contractile rate （

0.01）. The expression levels of α-SMA， desmin， collagen I， vimentin， phosphorylated JAK2 （p-JAK2）/JAK2， and phosphorylated STAT3 （p-STAT3）/STAT3 were significantly higher in the model group than in the normal group （

0.01）. The intervention group showed significantly lower expression levels of α-SMA， desmin， collagen I， vimentin， p-JAK2/JAK2， and p-STAT3/STAT3 than the model group （

0.01）.

Conclusion

QTC can promote phenotypic changes in aging CCSMCs and enhance their contractile function. The mechanism may be related to the modulation of the JAK2/STAT3 signaling pathway.

关键词

难治性勃起功能障碍衰老相关勃起功能障碍芪蛭通窍胶囊阴茎海绵体平滑肌细胞收缩功能Janus激酶2/信号转导和转录激活因子3信号通路

Keywords

refractory erectile dysfunctionaging-related erectile dysfunctionQizhi Tongqiao Capsulescorpus cavernosum smooth muscle cellscontractile functionJAK2/STAT3 signaling pathway

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