基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制

李林仙; 范子怡; 黄凯歌; 雷胜屹; 马胜南; 杨萍萍; 张萱; 孙占学

doi:10.16025/j.1674-1307.2025.11.004

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基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制

专题——特应性皮炎的中医临床与基础研究 | 更新时间：2026-01-29

- 基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制
- Mechanisms of Liangxue Qushi Zhiyang Formula in treatment of atopic dermatitis in mice based on mitochondrial morphology and fission
- 北京中医药 2025年44卷第11期页码：1390-1396
- 作者机构：
  
  北京中医药大学第三附属医院皮肤科，北京 100029
- 作者简介：
  
  李林仙，女，28岁，博士研究生。研究方向：中医药防治特应性皮炎。
  孙占学，E-mail：sunzhanxue@163.com
- 基金信息：
  
  国家自然科学基金面上项目(82274535);中华中医药学会2024年度基于国省标中药配方颗粒临床研究（脾胃科、皮肤科）专项(ZA_CACM_2024004);国家中医药管理局第五批全国中医临床优秀人才研修项目(国中医药人教函〔2022〕1号);北京中医药大学“岐黄英才·名医培育计划”(Y2023A05);北京中医药大学第三附属医院2022年度院级培育项目(BZYSY-2022-PYMS-12)
- DOI：10.16025/j.1674-1307.2025.11.004
  中图分类号：
- 收稿：2025-08-18，
  
  纸质出版：2025-11-25
- 稿件说明：
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李林仙，范子怡，黄凯歌，等.基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制［J］.北京中医药，2025，44（11）：1390-1396.

LI Linxian,FAN Ziyi,HUANG Kaige,et al.Mechanisms of Liangxue Qushi Zhiyang Formula in treatment of atopic dermatitis in mice based on mitochondrial morphology and fission[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(11):1390-1396.
李林仙，范子怡，黄凯歌，等.基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制［J］.北京中医药，2025，44（11）：1390-1396. DOI： 10.16025/j.1674-1307.2025.11.004.

LI Linxian,FAN Ziyi,HUANG Kaige,et al.Mechanisms of Liangxue Qushi Zhiyang Formula in treatment of atopic dermatitis in mice based on mitochondrial morphology and fission[J]. Beijing Journal of Traditional Chinese Medicine,2025,44(11):1390-1396. DOI： 10.16025/j.1674-1307.2025.11.004.

摘要

目的

基于线粒体形态和分裂探讨凉血祛湿止痒方治疗特应性皮炎小鼠的作用机制。

方法

采用随机数字表法将32只小鼠分为正常组、模型组、中药组和阳性药物（对照）组，各8只。除正常组外，其他各组用2，4-二硝基氯苯（DNCB）制备特应性皮炎模型。中药组予凉血祛湿止痒方生药5.2 g/（kg·d），对照组予氯雷他定片13.13 mg/（kg·d），正常组和模型组予等体积生理盐水，每天分2次灌胃，疗程1周。治疗前后采用湿疹面积及严重程度指数（EASI）评分对皮损进行评价，统计小鼠30 min内的抓挠次数，苏木精-伊红（HE）染色观察背部皮肤组织病理学改变，透射电镜观察胸腺细胞线粒体超微结构，蛋白免疫印迹（WB）法检测皮损组织动力相关蛋白1（Drp1）、受线粒体分裂因子（Mff）、裂变蛋白1（Fis1）表达。

结果

与正常组比较，模型组小鼠在治疗前后背部皮肤红斑、丘疹/水肿、表皮剥脱、苔藓化以及总分均高（

0.01），搔抓次数多（

0.01）；组织病理提示表皮增厚，炎症细胞浸润；电镜下观察小鼠胸腺细胞线粒体结构呈水肿、空泡状，嵴断裂、溶解；皮损组织中Drp1、Mff、Fis1蛋白表达高（

0.01）。治疗后，与模型组比较，中药组小鼠各项皮损评分及总分均低（

0.01），搔抓次数少（

0.05），表皮增厚及炎症浸润均改善；小鼠胸腺细胞线粒体结构基本正常，嵴较致密，管腔狭窄细长，结构较清晰；皮损组织中Drp1、Mff和Fis1蛋白表达均低（

0.01）。治疗后，对照组的有效率为37.5%，中药组为87.5%。

结论

凉血祛湿止痒方可改善特应性皮炎小鼠的皮损、瘙痒和组织病理改变，其作用机制可能与抑制Drp1、Mff、Fis1等线粒体分裂相关蛋白表达，调节线粒体形态有关。

Abstract

Objective

To investigate the mechanisms of

Liangxue Qushi Zhiyang Formula

in the treatment of atopic dermatitis in mice based on mitochondrial morphology and fission.

Methods

Thirty-two mice were randomly divided into normal group， model group， Chinese medicine group， and positive drug （control） group， with 8 mice in each group. Except for the normal group， atopic dermatitis was induced in all groups using 2，4-dinitrochlorobenzene （DNCB）. The Chinese medicine group was administered

Liangxue Qushi Zhiyang Formula

at a crude drug dose of 5.2 g/（kg•d）， the control group was administered loratadine tablets at a dose of 13.13 mg/（kg•d）， and the normal and model groups were given an equal volume of normal saline. All treatments were administered by gavage twice daily for 1 week. Before and after treatment， skin lesions were evaluated using the Eczema Area and Severity Index （EASI） score， and the number of scratching episodes within 30 min was recorded. Histopathological changes in dorsal skin tissues were observed by hematoxylin-eosin （HE） staining. The ultrastructure of mitochondria in thymic cells was examined by transmission electron microscopy. Protein expression levels of dynamin-relat

ed protein 1 （Drp1）， mitochondrial fission factor （Mff）， and fission protein 1 （Fis1） in skin lesion tissues were detected by Western blot.

Results

Compared with the normal group， mice in the model group showed significantly higher scores for dorsal skin erythema， papules/edema， epidermal exfoliation， lichenification， and total score both before and after treatment（

0.01）， as well as significantly increased scratching episodes（

0.01）. Histopathological examination showed epidermal thickening and inflammatory cell infiltration. Transmission electron microscopy revealed mitochondrial edema and vacuolization， with disrupted and dissolved cristae in thymic cells. The expression levels of Drp1， Mff， and Fis1 proteins in skin lesion tissues were significantly increased（

0.01）. After treatment， compared with the model group， the Chinese medicine group showed significantly lower scores for all skin lesion items and total score（

0.01）， as well as fewer scratching episodes（

0.05）. Epidermal thickening and inflammatory infiltration were improved. Mitochondrial structure in thymic cells was basically normal， with relatively dense cristae， narrow and elongated lumens， and clearer structures. The expression levels of Drp1， Mff， and Fis1 proteins in skin lesion tissues were significantly decreased（

0.01）. After treatment， the effective rate was 37.5% in the control group and 87.5% in the Chinese medicine group.

Conclusion

Liangxue Qushi Zhiyang Formula

can improve skin lesions， pruritus， and histopathological changes in mice with atopic dermatitis. Its mechanisms may be related to the inhibition of mitochondrial fission-related proteins such as Drp1， Mff， and Fis1， thereby regulating mitochondrial morphology.

关键词

Keywords

references

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