针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究

欧宇阳; 李丹; 曾学翰; 李雨农; 罗耀星; 张艺馨

doi:10.16025/j.1674-1307.2026.03.013

您当前的位置：

首页 >

文章列表页 >

针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究

实验研究 | 更新时间：2026-06-02

- 针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究
- Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage
- 北京中医药 2026年45卷第3期页码：358-365
- 作者机构：
  
  北京中医药大学第三附属医院针灸科，北京 100029
- 作者简介：
  
  欧宇阳，男，28岁，博士研究生。研究方向：针刺治疗脑病。
  李丹，E-mail：3509437@qq.com
- 基金信息：
  
  国家自然科学基金面上项目(82174514)
- DOI：10.16025/j.1674-1307.2026.03.013
  中图分类号：
- 收稿：2024-12-20，
  
  纸质出版：2026-03-25
- 稿件说明：
移动端阅览
欧宇阳，李丹，曾学翰，等.针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究［J］.北京中医药，2026，45（3）：358-365.

OU Yuyang,LI Dan,ZENG Xuehan,et al.Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(03):358-365.
欧宇阳，李丹，曾学翰，等.针刺百会透曲鬓对急性脑出血大鼠脑组织线粒体动力学的影响及机制研究［J］.北京中医药，2026，45（3）：358-365. DOI： 10.16025/j.1674-1307.2026.03.013.

OU Yuyang,LI Dan,ZENG Xuehan,et al.Effects and mechanisms of acupuncture at Baihui (GV20) penetrating Qubin (GB7) on mitochondrial dynamics in brain tissue of rats with acute intracerebral hemorrhage[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(03):358-365. DOI： 10.16025/j.1674-1307.2026.03.013.

摘要

目的

探讨针刺百会透曲鬓对脑出血（ICH）大鼠线粒体动力学的影响及机制。

方法

将30只大鼠用随机数字表法分为假手术组（A组）、模型组（B组）、针刺组（C组）、针刺+泛素样蛋白4A（UBL4A）敲低阴性对照组（D组）、针刺+UBL4A敲低组（E组），每组6只。其中D组、E组于建模前3 d进行立体定向病毒注射。除A组外，其他各组采用自体血注入法制备ICH-右侧纹状体大鼠模型。造模1 h后，A组、B组仅捆绑，每天1次，每次30 min，连续3 d。C组、D组、E组进行针刺治疗，连续3 d。3 d后处死大鼠，收集脑组织进行后续检测。对比各组Berderson神经功能评分，用苏木精-伊红（HE）染色观察脑组织病理变化，用实时荧光定量逆转录聚合酶链反应（RT-qPCR）检测脑组织UBL4A mRNA表达，用Western Blotting法检测脑组织Ser637位点磷酸化动力蛋白相关蛋白1［p-Drp1（Ser637）］、线粒体分裂蛋白1（Fis1）、线粒体融合蛋白1（Mfn1）蛋白表达。

结果

与A组比较，B组Berderson神经功能评分高（

0.01）；与B组比较，C组评分低（

0.01）；与C组比较，D组神经功能评分差异无统计学意义（

0.05），E组Berderson神经功能评分高（

0.01）。B组大鼠脑组织存在水肿及大量炎症细胞浸润；与B组比较，C组、D组大鼠脑组织水肿减轻，炎症细胞浸润少；E组脑组织水肿、炎症细胞浸润较B组减轻，但较C组、D组重。与A组比较，B组UBL4A mRNA表达低（

0.01）；与B组比较，C组UBL4A mRNA表达低高（

0.01）；与C组比较，D组UBL4A mRNA表达差异无统计学意义（

0.05），E组UBL4A mRNA表达低（

0.01）。与A组比较，B组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达低，而Fis1蛋白表达高（

0.01）；与B组比较，C组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达高，而Fis1蛋白表达低（

0.01）；与C组比较，D组大鼠脑组织中p-Drp1（Ser637）、Fis1和Mfn1蛋白表达差异无统计学意义（

0.05）；与C组比较，E组大鼠脑组织中p-Drp1（Ser637）、Mfn1蛋白表达低，而Fis1蛋白表达高（

0.01）。

结论

针刺百会透曲鬓可能通过调控UBL4A表达抑制ICH大鼠线粒体过度分裂，促进线粒体融合，有助于维持线粒体动力学平衡，从而减轻ICH后神经损伤。

Abstract

Objective

To investigate the effects of acupuncture at

Baihui

（GV20） penetrating

Qubin

（GB7） on mitochondrial dynamics in rats with intracerebral hemorrhage （ICH） and its underlying mechanisms.

Methods

Thirty rats were randomly divided into five groups using a random number table： sham operation group （Group A）， model group （Group B）， acupuncture group （Group C）， acupuncture + UBL4A knockdown negative control group （Group D）， and acupuncture + UBL4A knockdown group （Group E）， with 6 rats in each group. Groups D and E received stereotactic viral injections 3 days before modeling. Except for Group A， the ICH model was established in the remaining groups by autologous blood injection into the right striatum. One hour after modeling， Groups A， B， and C were only restrained and received no other intervention， once daily for 30 min for 3 consecutive days. Groups C， D， and E received acupuncture treatment for 3 consecutive days. Rats were sacrificed after 3 days， and brain tissue was collected for further analysis. Neurological function was evaluated using the Bederson score. Hematoxylin-eosin （HE） staining was used to observe histopathological changes in brain tissue. RT-qPCR was performed to detect UBL4A mRNA expression in brain tissue. Western blot was used to detect the expression of phosphorylated dynamin-related protein 1 at Ser637 ［p-Drp1 （Ser637）］， mitochondrial fission protein 1 （Fis1）， and mitochondrial fusion protein 1 （Mfn1）.

Results

Compared with Group A， Bederson scores in Group B were significantly increased （

0.01）. Compared with Group B， Group C showed significantly lower scores （

0.01）. Compared with Group C， there was no significant difference in neurological scores in Group D （P

0.05）， while Group E showed significantly higher scores （

0.01）. Brain tissue in Group B showed edema and extensive inflammatory cell infiltration. Compared with Group B， Groups C and D showed reduced brain edema and fewer inflammatory infiltrates. In Group E， brain edema and inflammatory infiltration were alleviated compared with Group B but were more severe than those in Groups C and D. Compared with Group A， UBL4A mRNA expression in Group B was significantly decreased （

0.01）. Compared with Group B， Group C showed significantly increased UBL4A mRNA expression （

0.01）. Compared with Group C， there was no significant difference in Group D （

0.05）， while Group E showed significantly decreased UBL4A mRNA expression （

0.01）. Compared with Group A， p-Drp1 （Ser637） and Mfn1 protein expression in Group B were significantly decreased， while Fis1 expression was significantly increased （

0.01）. Compared with Group B， Group C showed increased p-Drp1 （Ser637） and Mfn1 expression and decreased Fis1 expression （

0.01）. Compared with Group C， there were no significant differences in p-Drp1 （Ser637）， Fis1， and Mfn1 expression in Group D （

0.05）. Compared with Group C， Group E showed decreased p-Drp1 （Ser637） and Mfn1 expression and increased Fis1 expression （

0.01）.

Conclusion

Acupuncture at

Baihui

（GV20） penetrating

Qubin

（GB7） may regulate UBL4A expression to inhibit excessive mitochondrial fission and promote mitochondrial fusion in ICH rats， thereby maintaining mitochondrial dynamic balance and alleviating neurological injury after ICH.

关键词

Keywords

references

中华医学会神经病学分会 , 中华医学会神经病学分会脑血管病学组 . 中国脑出血诊治指南（2019） [J ] . 中华神经科杂志 , 2019 , 52 ( 12 ): 994 - 1005 .

龙昌友 , 曹云太 , 鲍海华 . 高血压脑出血后血肿周围水肿相关影响因素的研究进展 [J ] . 放射学实践 , 2024 , 39 ( 5 ): 704 - 710 .

张钟圆 . PGC-1α通过调控线粒体TOM70/MICU1通路在脑出血中的神经保护作用研究 [D ] . 杭州 : 浙江大学 , 2020 .

简丹 , 郑淑月 , 翟瑄 , 等 . 线粒体动力相关蛋白Drp1在小鼠脑出血后炎症反应中的作用 [J ] . 中国免疫学杂志 , 2023 , 39 ( 12 ): 2465 - 2469,2476 .

赵振荣 , 史洺 , 刘继红 , 等 . 线粒体动力相关蛋白1与帕金森病相关性研究 [J ] . 中国比较医学杂志 , 2022 , 32 ( 6 ): 117 - 121 .

谭子璇 , 朱武政 . 线粒体动力学调控机制及其在肾脏病理生理学中的作用 [J ] . 生理学报 , 2024 , 76 ( 1 ): 148 - 160 .

苑喜微 , 南月敏 . 线粒体融合蛋白2的结构和功能及其在肝脏疾病中作用机制的研究进展 [J ] . 中国全科医学 , 2023 , 26 ( 30 ): 3841 - 3846 .

CHEN H , LI L , HU J , et al . UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1 [J ] . J Exp Clin Cancer Res , 2019 , 38 ( 1 ): 297 .

HUANG S , LI Y , YUAN X , et al . The UbL-UBA Ubiquilin4 protein functions as a tumor suppressor in gastric cancer by p53-dependent and p53-independent regulation of p21 [J ] . Cell Death Differ , 2019 , 26 ( 3 ): 516 - 530 .

LI J , TONG Y , WANG Z , et al . A Comprehensive Multiomics Analysis Identified Ubiquilin 4 as a Promising Prognostic Biomarker of Immune-Related Therapy in Pan-Cancer [J ] . J Oncol , 2021 , 2021 : 7404927 .

ZHENG T , YANG Y , CASTAÑEDA CA . Structure, dynamics and functions of UBQLNs: at the crossroads of protein quality control machinery [J ] . Biochem J , 2020 , 477 ( 18 ): 3471 - 3497 .

王少松 , 李彬 , 孙敬青 , 等 . 针刺治疗急性缺血性脑卒中机制研究进展 [J ] . 北京中医药 , 2024 , 43 ( 1 ): 97 - 102 .

李龙 , 李丹 , 朱曦 , 等 . 针刺百会透曲鬓对脑内出血大鼠脑组织CD32、CD206蛋白表达水平的影响 [J ] . 上海针灸杂志 , 2024 , 43 ( 5 ): 561 - 568 .

ZOU W ， CHEN Q X ， SUN X W ， et al . Acupuncture inhibits Notch1 and Hes1 protein expression in the basal Ganglia of rats with cerebral hemorrhage [J ] . Neural Regeneration Research ， 2015 ， 10 ( 3 )： 457 - 462 .

王敏 , 贾敏 , 张昕洋 , 等 . 针刺治疗高血压性脑出血疗效及安全性的系统评价与Meta分析 [J ] . 中国中药杂志 , 2021 , 46 ( 18 ): 4644 - 4653 .

姚嘉永 , 邹伟 . 针刺治疗脑出血机制的研究进展 [J ] . 针刺研究 , 2022 , 47 ( 1 ): 88 - 94

李丹 , 朱曦 , 王中鹏 , 等 . 针刺"百会"透"曲鬓"对大鼠脑出血后小胶质细胞M1/M2型极化的影响 [J ] . 中国中西医结合杂志 , 2023 , 43 ( 11 ): 1359 - 1365 .

CHEN W , GUO C , FENG H , et al . Mitochondria: Novel Mechanisms and Therapeutic Targets for Secondary Brain Injury After Intracerebral Hemorrhage [J ] . Front Aging Neurosci , 2020 , 12 : 615451 .

ZERIHUN M , SUKUMARAN S , QVIT N . The Drp1-Mediated Mitochondrial Fission Protein Interactome as an Emerging Core Player in Mitochondrial Dynamics and Cardiovascular Disease Therapy [J ] . Int J Mol Sci , 2023 , 24 ( 6 ): 5785 .

JIN JY , WEI XX , ZHI XL , et al . Drp1-dependent mitochondrial fission in cardiovascular disease [J ] . Acta Pharmacol Sin , 2021 , 42 ( 5 ): 655 - 664 .

GUPTA A , BECKER T . Mechanisms and pathways of mitochondrial outer membrane protein biogenesis [J ] . Biochim Biophys Acta Bioenerg , 2021 , 1862 ( 1 ): 148323 .

STAVROPOULOS F , GEORGIOU E , SCHIZA N , et al . Mitofusin 1 overexpression rescues the abnormal mitochondrial dynamics caused by the Mitofusin 2 K357T mutation in vitro [J ] . J Peripher Nerv Syst , 2023 , 28 ( 3 ): 329 - 340 .

MARIAPPAN M , LI X , STEFANOVIC S , et al . A ribosome-associating factor chaperones tail-anchored membrane proteins [J ] . Nature , 2010 , 466 ( 7310 ): 1120 - 1124 .

PENG SJ , YAO RR , YU SS , et al . UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6 [J ] . J Immunol , 2019 , 203 ( 7 ): 1943 - 1951 .

TSCHOE C , BUSHNELL CD , DUNCAN PW , et al . Neuroinflammation after Intracerebral Hemorrhage and Potential Therapeutic Targets [J ] . J Stroke , 2020 , 22 ( 1 ): 29 - 46 .

刘泽 , 张硕 , 徐欣雨 , 等 . 基于线粒体自噬PINK1/Parkin信号通路的枳实、麸炒枳实和烫枳实对功能性消化不良大鼠治疗作用比较 [J ] . 北京中医药 , 2024 , 43 ( 8 ): 880 - 885 .

ZHANG H , ZHAO Y , YAO Q , et al . Ubl4A is critical for mitochondrial fusion process under nutrient deprivation stress [J ] . PLoS One , 2020 , 15 ( 11 ): e0242700 .

LI D , ZHAO Y , BAI P , et al . Baihui (DU20)-penetrating-Qubin (GB7) acupuncture regulates microglia polarization through miR-34a-5p/Klf4 signaling in intracerebral hemorrhage rats [J ] . Exp Anim , 2021 , 70 ( 4 ): 469 - 478 .

LI D , WANG L , SHI S , et al . Ubiquitin-like 4A alleviates the progression of intracerebral hemorrhage by regulating oxidative stress and mitochondrial damage [J ] . Exp Anim , 2024 , 73 ( 4 ): 421 - 432 .

TAI Y , PU M , YUAN L , et al . miR-34a-5p regulates PINK1-mediated mitophagy via multiple modes [J ] . Life Sci , 2021 , 276 : 119415 .

PICKLES S , VIGIÉ P , YOULE RJ . Mitophagy and Quality Control Mechanisms in Mitochondrial Maintenance [J ] . Curr Biol , 2018 , 28 ( 4 ): R170 - R185 .

BHATIA D , CAPILI A , CHOI ME . Mitochondrial dysfunction in kidney injury, inflammation, and disease: Potential therapeutic approaches [J ] . Kidney Res Clin Pract , 2020 , 39 ( 3 ): 244 - 258 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

心衰合剂对心肌梗死后心力衰竭大鼠PGC-1α/FNDC5信号通路及线粒体动力学的影响

旋提手法联合针刺治疗颈源性头痛的临床研究

基于脏腑经络学说探讨脑-甲状腺-肠轴与溃疡性结肠炎合并焦虑抑郁发病的关系

针刺调节心脾两虚型慢性失眠症过度觉醒状态的临床研究

针刺联合骨科熥药对膝骨关节炎寒湿痹阻证患者步态特征的影响