二陈汤对血脂异常痰浊阻遏证小鼠PHGDH甲基化及其介导的丝氨酸代谢信号通路的影响
Effects of
Erchen Decoction on PHGDH methylation and the PHGDH-mediated serine metabolism signaling pathway in mice with dyslipidemia of the phlegm-dampness obstruction syndrome- 北京中医药 2026年45卷第2期 页码：190-195
- 作者机构：
1.北京中医药大学中医学院，北京 102401
2.北京中医药大学东直门医院骨伤科，北京 100700
3.北京中医药大学国家中医体质与治未病研究院，北京 102401
4.北京中医药大学第三附属医院治未病中心，北京 100029
- 作者简介：
陈心怡，女，25岁，硕士研究生。研究方向：血脂异常不同证候的基础研究及分子生物学研究。
陈婧，E-mail：jchen@bucm.edu.cn
- 基金信息：北京市自然科学基金面上项目(7242233);国家自然科学基金青年科学基金项目(82004237);北京中医药大学中央高校基本科研业务费（揭榜挂帅）项目(2023-JYB-JBQN-044);北京中医药大学第三附属医院精诚人才（精诚青苗）项目(2025-QNKY-002);北京中医药新时代125工程培训项目(京中医药科字〔2025〕2号);北京高层次创新创业人才支持计划（春蕾）项目(G202534231);中央高水平中医医院临床科研业务费项目(DZMG-ZLZX-25021)
DOI：10.16025/j.1674-1307.2026.02.007
中图分类号：收稿：2025-04-11，
纸质出版：2026-02-25
- 稿件说明：
移动端阅览
陈心怡，薛晓琳，叶超，等.二陈汤对血脂异常痰浊阻遏证小鼠PHGDH甲基化及其介导的丝氨酸代谢信号通路的影响［J］.北京中医药，2026，45（2）：190-195.
CHEN Xinyi,XUE Xiaolin,YE Chao,et al.Effects of Erchen Decoction on PHGDH methylation and the PHGDH-mediated serine metabolism signaling pathway in mice with dyslipidemia of the phlegm-dampness obstruction syndrome[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(02):190-195.
陈心怡，薛晓琳，叶超，等.二陈汤对血脂异常痰浊阻遏证小鼠PHGDH甲基化及其介导的丝氨酸代谢信号通路的影响［J］.北京中医药，2026，45（2）：190-195. DOI： 10.16025/j.1674-1307.2026.02.007.
CHEN Xinyi,XUE Xiaolin,YE Chao,et al.Effects of Erchen Decoction on PHGDH methylation and the PHGDH-mediated serine metabolism signaling pathway in mice with dyslipidemia of the phlegm-dampness obstruction syndrome[J]. Beijing Journal of Traditional Chinese Medicine,2026,45(02):190-195. DOI： 10.16025/j.1674-1307.2026.02.007.
摘要
目的
2
观察二陈汤对血脂异常痰浊阻遏证小鼠3-磷酸甘油酸脱氢酶（PHGDH）甲基化及其介导的丝氨酸代谢信号通路的影响。
方法
2
将C57BL/6J小鼠作为正常组，取ApoE基因敲除小鼠20只制备血脂异常痰浊阻遏证模型，取5只制备血脂异常脾肾阳虚证模型。造模成功后，用随机数字表法将ApoE基因敲除小鼠分为模型组、方证相应低剂量组、方证相应中剂量组、方证相应高剂量组、方证不相应组，每组各5只。方证相应低、中、高剂量组分别给予二陈汤4.45、8.90、17.8 g/（kg·d）灌胃；方证不相应组给予二陈汤8.90 g/（kg·d）灌胃；正常组、模型组给予相同体积的生理盐水灌胃。均每天灌胃1次，共4周。用酶联免疫吸附试验（ELISA）检测血清PHGDH水平，甲基化敏感限制性内切酶聚合酶链反应（MSRE-PCR）检测主动脉PHGDH基因甲基化水平，逆转录定量聚合酶链反应（RT-PCR）检测主动脉PHGDH mRNA表达水平，液相色谱-质谱联用（LC-MS）法检测血清丝氨酸代谢通路关键代谢物水平。
结果
2
与正常组比较，模型组小鼠PHGDH基因甲基化水平高，PHGDH mRNA表达、血清PHGDH水平低（
P
<
0.05）；与模型组比较，方证相应中剂量组PHGDH基因甲基化水平低，PHGDH mRNA表达、血清PHGDH水平、丝氨酸、苏氨酸水平高（
P
<
0.05）；与方证相应中剂量组比较，方证不相应组PHGDH基因甲基化水平高，PHGDH mRNA表达、血清PHGDH水平、丝氨酸、苏氨酸水平低（
P
<
0.05）。
结论
2
二陈汤可抑制血脂异常痰浊阻遏证小鼠主动脉PHGDH基因甲基化，从而提高PHGDH基因表达水平，降低氧化应激，激活丝氨酸代谢通路。
Abstract
Objective
2
To investigate the effects of
Erchen Decoction
on 3-phosphoglycerate dehydrogenase （PHGDH） methylation and the PHGDH-mediated serine metabolism signaling pathway in mice with dyslipidemia phlegm-dampness obstruction syndrome.
Methods
2
C57BL/6J mice were used as the normal group. Twenty ApoE knockout mice were selected using a random number table method to establish a model of dyslipidemia with phlegm-dampness obstruction syndrome， and five ApoE knockout mice were used to establish a model of dyslipidemia with spleen-kidney
yang
deficiency syndrome. After successful modeling， the ApoE knockout mice were randomly divided into a model group， a prescription-syndrome corresponding low-dose group， a prescription-syndrome corresponding medium-dose group， a prescription-syndrome corresponding high-dose group， and a prescription-syndrome non-corresponding group， with 5 mice in each group. The prescription-syndrome corresponding low-， medium-， and high-dose groups were administered
Erchen Decoction
by gavage at doses of 4.45， 8.90， and 17.8 g/（kg·d）， respectively. The prescription-syndrome non-corresponding group received
Erchen Decoction
at 8.90 g/（kg·d）. The normal and model groups were given an equal volume of normal saline by gavage. All groups were treated once daily for 4 weeks. Serum PHGDH levels were measured by enzyme-linked immunosorbent assay （ELISA）. The methylation level of the PHGDH gene in aortic tissue was determined by methylation-sensitive restriction endonuclease polymerase chain reaction （MSRE-PCR）. The mRNA expression level of PHGDH in aortic tissue was measured by reverse transcription polymerase chain reaction （RT-PCR）. Serum levels of key metabolites in the serine metabol
ism pathway were detected by liquid chromatography-mass spectrometry （LC-MS）.
Results
2
Compared with the normal group， the model group showed higher methylation levels of the PHGDH gene and lower PHGDH mRNA expression and serum PHGDH levels （
P
<
0.05）. Compared with the model group， the prescription-syndrome corresponding medium-dose group showed lower PHGDH gene methylation levels and higher PHGDH mRNA expression， serum PHGDH levels， and levels of serine and threonine （
P
<
0.05）. Compared with the prescription-syndrome corresponding medium-dose group， the prescription-syndrome non-corresponding group showed higher PHGDH gene methylation levels and lower PHGDH mRNA expression， serum PHGDH levels， and levels of serine and threonine （
P
<
0.05）.
Conclusion
2
Erchen Decoction
can inhibit PHGDH gene methylation in the aorta of mice with dyslipidemia phlegm-dampness obstruction syndrome， thereby upregulating PHGDH gene expression， reducing oxidative stress， and activating the serine metabolism pathway.
关键词
Keywords
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