Effect of Wenshen Jianpi Tiaoshu Recipe on expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome rats with spleen and kidney Yang deficiency

SU Xiao-lan; ZHANG Tao; MAO Xin-yong; GUO Song; YANG Yang; WANG Xin; HOU Li-wei; WEI Wei

doi:10.16025/j.1674-1307.2021.03.007

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Effect of Wenshen Jianpi Tiaoshu Recipe on expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome rats with spleen and kidney Yang deficiency

更新时间：2023-04-10

- Effect of Wenshen Jianpi Tiaoshu Recipe on expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome rats with spleen and kidney Yang deficiency
- Beijing Journal of Traditional Chinese Medicine Vol. 40, Issue 3, Pages: 240-245(2021)
- 作者机构：
  
  1. 中国中医科学院望京医院脾胃病科
- 作者简介：
- 基金信息：
- DOI：10.16025/j.1674-1307.2021.03.007
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[1]苏晓兰,张涛,毛心勇,国嵩,杨洋,王欣,侯理伟,魏玮.温肾健脾调枢方对脾肾阳虚型腹泻型肠易激综合征大鼠GFAP、BDNF表达的影响[J].北京中医药,2021,40(03):240-245.

SU Xiao-lan, ZHANG Tao, MAO Xin-yong, et al. Effect of Wenshen Jianpi Tiaoshu Recipe on expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome rats with spleen and kidney Yang deficiency. [J]. Beijing Journal of Traditional Chinese Medicine 40(3):240-245(2021)
[1]苏晓兰,张涛,毛心勇,国嵩,杨洋,王欣,侯理伟,魏玮.温肾健脾调枢方对脾肾阳虚型腹泻型肠易激综合征大鼠GFAP、BDNF表达的影响[J].北京中医药,2021,40(03):240-245. DOI： 10.16025/j.1674-1307.2021.03.007.

SU Xiao-lan, ZHANG Tao, MAO Xin-yong, et al. Effect of Wenshen Jianpi Tiaoshu Recipe on expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome rats with spleen and kidney Yang deficiency. [J]. Beijing Journal of Traditional Chinese Medicine 40(3):240-245(2021) DOI： 10.16025/j.1674-1307.2021.03.007.

摘要

目的观察温肾健脾调枢方对脾肾阳虚型腹泻型肠易激综合征（diarrhea-predominant irritable bowel syndrome, IBS-D）大鼠胶质纤维酸性蛋白（GFAP）、脑源性神经营养因子（BDNF）表达的影响,从实验角度探讨该方对IBS-D的疗效及作用机制。方法 72只Wistar大鼠随机分为6组:正常组（NG）、模型组（MG）、中药高中低剂量组（WHG、WMG、WLG）和匹维溴铵对照组（CG）,每组12只。除NG外,其余各组均采用避水应激+番泻叶灌胃法建立脾肾阳虚型IBS-D大鼠模型。造模结束后,中药治疗组分别予温肾健脾调枢方高、中、低剂量灌胃,CG予匹维溴铵灌胃,MG、NG予等体积生理盐水灌胃,疗程10 d。观察各组大鼠排便粒数、内脏敏感性和组织病理学,免疫组化检测结肠组织中GFAP、BDNF表达。结果治疗结束,WHG、CG和WMG排便粒数显著少于MG （P<0.01）,且WHG少于CG （P<0.05）;60、80 mmHg时各组大鼠腹壁撤退反射（AWR）评分表现出差异,各治疗组AWR评分低于MG （P<0.05）,且WHG低于CG（P<0.05）;组织病理学观察MG和WLG黏膜可见轻度水肿;各治疗组大鼠结肠组织GFAP表达均低于MG（P<0.05）,且WMG低于CG（P<0.05）;WHG、WMG和CG结肠组织BDNF表达低于MG （P<0.05）,且WHG低于CG（P<0.05）。结论温肾健脾调枢方能够减少IBS-D大鼠排便粒数、降低内脏敏感性,其治疗机理可能是通过影响结肠组织中GFAP、BDNF的表达,进而起到调节内脏敏感性的作用。

Abstract

Objective To observe the effect of Wenshen Jianpi Tiaoshu Recipe on the expression of GFAP and BDNF in diarrhea-predominant irritable bowel syndrome（IBS-D）rats with spleen and kidney Yang deficiency, and to explore the therapeutic effect and mechanism of this recipe on IBS-D from the experimental point of view.Methods 72 Wistar rats were randomly divided into 6 groups: normal group（NG）,model group（MG）,Chinese medicine treatment group（WHG,WMG,WLG）and the control group（CG）,12 in each group.Apart from NG,the IBS-D rat model with spleen and kidney Yang deficiency in other groups were established with the method of avoiding water stress + senna leaf gavage.At the end of the modelling, Chinese medicine treatment groups were given high, medium and low doses of Wenshen Jianpi Tiaoshu Recipe, CG was gavaged with pinaverium bromide and MG and NG were given equal volume of normal saline by intragastric administration respectively, all for 10 days.The number of defecation pellets, visceral sensitivity and histopathology were observed.The expression of GFAP and BDNF in colon tissue was detected by immunohistochemistry.Results At the end of treatment, the number of defecation pellets of WHG,CG and WMG was significantly less than that of MG（P<0.01）,and WHG was less than CG（P<0.05）; The abdominal wall withdrawal reflex（AWR）scores of rats in each group at 60 and 80 mmHg showed difference, the AWR score of each treatment group was lower than MG（P<0.05）,and WHG of treatment group was lower than that of CG（P<0.05）and WHG was less than CG（P<0.05）;Mild edema was observed in MG and WLG mucosa by histopathology; GFAP expression of all treatment group was lower than that of MG（P< 0.05）,and WHG was lower than CG（P<0.05）; BDNF expression in WHG,WMG and CG colon tissue was lower than that in MG（P<0.05）,and WHG was lower than CG（P<0.05）.Conclusion Wenshen Jianpi Tiaoshu Recipe can reduce defecation pellets number and reduce visceral sensitivity in IBS-D rats.Its therapeutic mechanism may be reducing visceral sensitivity through affecting the expression of GFAP and BDNF in colon tissue.

关键词

腹泻型肠易激综合征温肾健脾脾肾阳虚大鼠

Keywords

Diarrhea-predominant irritable bowel syndrome（IBS-D）warming kidney and strengthening spleenYang deficiency of spleen and kidneyrat

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Department of Spleen and Stomach Diseases，Wangjing Hospital，China Academy of Chinese Medical Sciences，Beijing Key Laboratory of Traditional Chinese Medicine Diagnosis and Treatment on Functional Gastrointestinal Disorders

China Academy of Chinese Medical Sciences

Center for Evidence-based Chinese Medicine，Beijing University of Chinese Medicine

Department of Health Care for Cadres， Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical University

Spleen and Stomach Diseases Department， Yangzhou Hospital Affiliated to Nanjing University of Traditional Chinese Medicine

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